Isoelectric focusing sample injection for capillary zone electrophoresis in a fused silica capillary.
نویسندگان
چکیده
An improvement has been made to couple isoelectric focusing (IEF) sample injection and capillary zone electrophoresis in an untreated fused silica capillary. Electroosmotic flow is efficiently prevented by simply using a rubber block at the outlet end of the capillary during IEF sample injection. The experimental conditions that affect the concentration effect are discussed. A concentration enhancement factor of over 100-fold can be easily obtained for two model proteins: lysozyme and ribonuclease A.
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ورودعنوان ژورنال:
- Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
دوره 22 7 شماره
صفحات -
تاریخ انتشار 2006